Cover crops are a key component of conservation cropping systems. They can also be a key component of integrated crop-livestock systems by offering high-quality forage during short periods between cash crops. The impact of cattle grazing on biologically active soil carbon (C) and nitrogen (N) fractions has not received much attention. This study investigated the impacts of tillage (conventional disk and no tillage) and cover crop management (ungrazed and grazed) on biologically active soil C and N fractions from biennial sampling during seven years of continuous management.